亲本死亡时间对近太湖新银鱼人工授精受精率的影响

银鱼捕起后易死,较难用活的亲本进行人工繁殖,在实际生产中,多采用死的亲本进行人工受精,亲本死亡的时间对卵的受精率影响较大。本文对徐家河水库近太湖新银鱼亲本死亡时间与卵受精率的关系进行了系列研究,亲本死亡后240分钟内,每隔15分钟进行一次人工受精,结果表明,卵的受精率随着亲本死亡时间的延长,其受精率越来越低,240分钟后,受精率趋于0。水温在15℃以下,亲本死亡105分钟后进行人工受精,平均受精率为43%,因此建议水温在15℃以下近太湖新银鱼的人工受精应在亲本死亡后105分钟之内进行。  

THE EFFECT OF POST-DEAD DURATION OF BROODFISH ON ARTIFICIAL FERTILIZATION RATE OF NEOSALANS PSEUDOTAIHUENSIS

A series of experiments to study the effect of post-dead duration of the broodfish of N.pseudotaihuensis on fertility were carried out from February to May 1996 in Xujiahe Reservoir.The fertility was completely lost after 240 minutes of the dead of broodfish at the temperature of 10℃ .The post-dead duration of males effected fertility more greatly than that of females, and there was a significant interaction between them.Sperm and eggs could be prolonged the viability after short-term conservation at low temperature (0℃) .Artificial insemination of N.pseudotaihu-ensis should be conducted within 105 minutes after the dead of broodfish at the temperature of lower than 15℃ .