A one-minute oxidase test to detect Vibrio strains isolated from cultures on thiosulphate-citrate-bile salts-sucrose (TCBS) medium |
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| Authors: | J Vila S Abdalla J Gonzalez C Garcia JA Bombi MT Jimenez de Anta |
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| Institution: | Servei de Microbiologia i Parasitologia;Servei d'Anatomia Patológica, Hospital Clinic i Provincial de Barcelona, Facultat de Medicina, Universitat de Barcelona Villarroel, Barcelona, Spain;Department of Microbiology, Faculty of Medicine, Assiut University, Assiut, Egypt |
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| Abstract: | J. VILA, S. ABDALLA, J. GONZALEZ, C. GARCIA, J. A. BOMBI AND M.T. JIMENEZ. 1992. Vibrio cholerae is oxidase positive, a primary characteristic used to differentiate it from Enterobacteriaceae. But false negative oxidase test results have been obtained with colonies from thiosulphate-citrate-bile salts-sucrose (TCBS) agar medium. A rapid oxidase test procedure is described here. This takes 1 min, avoids false negative results and the necessity to grow the bacteria in a general-purpose medium. The bacteria may be recovered after the test and used for further investigations. |
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