Cutting edge: IL-1beta mediates the proangiogenic activity of osteopontin-activated human monocytes |
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Authors: | Naldini Antonella Leali Daria Pucci Annalisa Morena Emilia Carraro Fabio Nico Beatrice Ribatti Domenico Presta Marco |
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Affiliation: | Unit of Neuroimmunophysiology, Department of Physiology, University of Siena, Via Aldo Moro, 53100 Siena, Italy. Naldini@Unisi.it |
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Abstract: | Inflammation plays an important role in the onset of angiogenesis. In the present study, we show that osteopontin (OPN), a proinflammatory mediator involved in tissue repair, induces IL-1beta up-regulation in human monocytes. This was accompanied by the enhanced production of TNF-alpha, IL-8, and IL-6, a decreased release of IL-10, and increased p38 phosphorylation. The supernatants of OPN-treated monocytes were highly angiogenic when delivered on the chick embryo chorioallantoic membrane. The angiogenic response was completely abrogated by a neutralizing anti-IL-1 Ab, thus indicating that this cytokine represents the major proangiogenic factor expressed by OPN-activated monocytes. Accordingly, rIL-1beta mimicked the proangiogenic activity of OPN-treated monocyte supernatants, and IL-1R (type I) was found to be expressed in the chorioallantoic membrane. In conclusion, OPN-activated monocytes may contribute to the onset of angiogenesis through a mechanism mediated by IL-1beta. |
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