Isolation and primary cultures of human intrahepatic bile ductular epithelium |
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Authors: | A J Demetris B H Markus S Saidman J J Fung L Makowka S Graner R Duquesnoy T E Starzl |
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Institution: | (1) Department of Pathology, Presbyterian University Hospital, Pittsburgh, PA;(2) Department of Surgery, Presbyterian University Hospital, Pittsburgh, PA;(3) Department of Surgery, University of Rochester, NY |
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Abstract: | Summary A technique for the isolation of human intrahepatic bile ductular epithelium, and the establishment of primary cultures using
a serum- and growth-factor-supplemented medium combined with a connective tissue substrata is described. Initial cell isolates
and monolayer cultures display phenotypic characteristics of biliary epithelial cells (low molecular weight prekeratin positive;
albumin, alphafetoprotein, and Factor VIII-related antigen negative). Ultrastructural features of the cultured cells show
cell polarization with surface microvilli, numerous interepithelial junctional complexes and cytoplasmic intermediate prekeratin
filaments. |
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Keywords: | biliary epithelium bile duct cell culture human |
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