Studies of the biosynthesis of the cation-dependent mannose 6-phosphate receptor in murine cell lines |
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Authors: | S Gasa S Kornfeld |
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Institution: | 1. Institute for Lasers, Photonics and Biophotonics and the Department of Chemistry, University at Buffalo, The State University of New York, Buffalo, NY 14260, USA;2. Department of Rehabilitation Science, School of Public Health and Health Professions, University at Buffalo, The State University of New York, Buffalo, NY 14214, USA;3. Department of Pathology and Anatomical Science, Jacobs School of Medicine & Biomedical Sciences, University at Buffalo, 955 Main Street, Buffalo, NY 14203, USA;4. Department of Medicine, Division of Allergy, Immunology, and Rheumatology, State University of New York at Buffalo, Clinical Translational Research Center, Buffalo, NY 14203, USA;1. Marie-Curie Research Fellow, Institute for Infrastructure and Environment, Heriot-Watt University, UK;2. Institute for Infrastructure and Environment, Heriot-Watt University, UK;3. Department of Civil Engineering, University of Patras, Greece;4. Institute for Infrastructure and Environment, Heriot-Watt University, UK;1. Universidade Federal do Estado do Rio de Janeiro (UNIRIO), Rio de Janeiro, RJ, Brazil;2. Instituto de Psiquiatria da Universidade Federal do Rio de Janeiro (IPUB/UFRJ), Rio de Janeiro, RJ, Brazil;3. Pontifícia Universidade Católica do Rio de Janeiro (PUC-Rio), Rio de Janeiro, RJ, Brazil;4. Institute of Psychiatry, Psychology & Neuroscience, King''s College London, UK;5. Faculdade de Ciências Médicas da Universidade do Estado Do Rio de Janeiro (FCM/UERJ), Rio de Janeiro, RJ, Brazil |
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Abstract: | We have studied the biosynthesis of the cation-dependent mannose 6-phosphate receptor in murine BW5147 lymphoma cells and MOPC 315 plasmacytoma cells. The cells were labeled with 35S]methionine or 2-3H]mannose and the receptor immunoprecipitated with an anti-receptor antiserum. The receptor was first detected as a glycoprotein with an apparent molecular mass of 40 kDa. This intermediate was rapidly processed to a mature form which was stable during 22 h of chase. In these cells, the mature receptor has an apparent molecular mass of 43 kDa. The 3-kDa increase occurs as a result of processing of Asn-linked high-mannose oligosaccharides to complex-type units. |
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