烟草T-phylloplanin基因编码蛋白结构与功能的生物信息分析

利用美国国家生物技术信息中心(NCBI)网站所提供的相关信息,分析T-phylloplanin基因编码蛋白。该基因全长861hp,有一个完整的330hp的开放读框,编码110个氨基酸。该基因编码蛋白分子量为11.31kD,理论等电点为7.74。其氨基酸残基的不同区域分布有多N-糖基化位点、酪蛋白激酶Ⅱ磷酸化位点和N-肉豆蔻酰化位点,还有一个跨膜信号肽。T-phylloplanin基因编码蛋白与烟草叶片短柄腺毛分泌的抗性蛋白具有高度的同源性(93%),显示它在烟草抗性系统研究中有潜在价值。

Analysis of Structure and Function of the Protein Encoded by T-phylloplanin Gene from Nicotiana tabacum

The T-phylloplanin encoding protein was analysed by informatics tools provided on internet. The entirely T-phylloplanin gene has 861 bp containing a complete open reading frame that encodes 111 amino acids with a transmembrane signal peptide,several N-glycosylation and casein kinase II phosphorylation sites. The molecular weight of the protein encoded by T-phy lloplhin gene is 11.30 kD,and iso-electric point is 7.74. The T-phylloplanin encoding protein has highest similarity with the protein featuring defensive function secreted by short stalk glandular hair,thus indicating the worth for further study in tobacco resistance system.