Quantitative analysis of calcium gradients and activity in growing pollen tubes ofLilium longiflorum |
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Authors: | R. Nobiling H. -D. Reiss |
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Affiliation: | (1) Zellenlehre, Universität Heidelberg, Deutschland;(2) I. Physiologisches Institut, Universität Heidelberg, Im Neuenheimer Feld 326, D-6900 Heidelberg, Germany |
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Abstract: | Summary Pollen tubes ofLilium longiflorum were loaded with quin-2 to determine the cytoplasmic free calcium. Quin-2-fluorescence was detected at 500 nm with alternating excitation at 340 nm and 360 nm. The calcium2+-concentration was obtained using the intensity ratio R=I340/I360. The analysis exhibits a [Ca2+] of nearly 10–7mol·l–1 in the tip region and about 2·10–8 mol·l–1at the tube base, near the pollen grain. The data give evidence for the existence of a continuous gradient of free calcium within growing pollen tubes of various length. |
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Keywords: | Calcium activity Calcium gradient Lilium Polar growth Pollen tube Quin-2 Microfluorimeter |
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