Self-association of hemoglobin: a dielectric dispersion study

The concentration dependence of the dielectric dispersion between 1 kHz and 0.5 MHz was investigated for dense hemoglobin solutions. The concentration was varied between 4 and 26 mM/l. per heme. From the characteristic changes in dielectric parameters at some threshold concentration, we observe that the association of hemoglobin macromolecules occurs. This statement is also confirmed by viscosity measurement data.