野艾蒿及其有机提取物对铜绿微囊藻生长的抑制作用

通过植物与藻共培养实验,比较3种常见陆生菊科植物野艾蒿(Artemisia lavandulaefolia)、小白酒草(Conyza canadensis)、杭白菊(Chrysanthemum morifolium)的抑藻能力。结果表明野艾蒿和小白酒草能够适应水培,野艾蒿和杭白菊(后期为残体)抑藻能力较强,10d后对起始藻浓度为2×106cells/ml的铜绿微囊藻(Microcystic aeruginosa)生长的抑制率分别为93.3%和90.8%,对4×106cells/ml铜绿微囊藻抑制率分别为89.3%和79.2%。利用3种极性不同的有机溶剂(乙醇、正己烷、乙酸乙酯)提取野艾蒿干粉中的抑藻活性物质并进行生物检测,结果显示挥干溶剂后3种提取物都有较强抑藻能力,其中乙酸乙酯提取物抑藻能力最强,用线性拟合得到0.83g/L提取物72h后抑藻率达100%。研究表明,陆生菊科植物具有抑藻效果,野艾蒿具有应用于水体富营养化的治理和开发新型抑藻剂的潜力。

Inhibitive effects on Microcystis aeruginosa by Artemisia lavandulaefoli and its three organic solvents extracts

the inhibitive effects of three terrestrial Compositae plants, Artemisia lavandulaefolia, Conyza Canadensis and Chrysanthemum morifolium on the blue algae were compared by co-cultivation with Microcystic aeruginosa. Results showed that Artemisia lavandulaefolia and Conyza canadensis accommodate to water cultivation, Artemisia lavandulaefolia and Chrysanthemum morifolium (rotted during the last three days) had the most inhibitive potential, with the inhibition ratios to algae (inoculation concentration 2.0 × 10~6 cells/ml) is 93.3% and 90.8% respectively, to algae (inoculation concentration 4.0 10~6 cells/ml)is 89.3% and 79.2% on the 10~(th) day. The active compounds from dry powder of Artemisia lavandulaefolia were extracted by three kinds of organic solvents with different polarities and all the three extracts (solvents removed) had significant inhibitive effect. Among them, ethyl acetate extract was the strongest, with 0.83g/L extract that will reach up to 100% inhibition ratio by calculation. Result showed that A. lavandulaefolia, which grew well in the aquatic environment, might have a great potential to be applied to treat algae bloom and control the eutrophication of water.