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| HIV-1 Gag pl7-p24在痘苗病毒中的表达及性质研究 | |
| 引用本文： | 李体远,金宁一,王宏伟,郭志儒,方厚华,安汝国,殷震. HIV-1 Gag pl7-p24在痘苗病毒中的表达及性质研究[J]. 生物工程学报, 2000, 16(1): 65-68 |
| 作者姓名： | 李体远 金宁一 王宏伟 郭志儒 方厚华 安汝国 殷震 |
| 作者单位： | 1. 深圳市人民医院中心实验室 2. 解放军农牧大学研究所病毒室,长春,130062 3. 白求恩医科大学,长春,130021 |
| 基金项目： | 国家自然科学基金(39770661)和国家杰出青年基金(39825119)项目 |
| 摘 要： | 研究了重组痘苗病毒表达的ＨＩＶ－１核心蛋白（Ｇａｇ）ｐ１７－ｐ２４蛋白的些生物学及免疫学特点。间接免疫荧光、Ｄｏｔ 及ＬＩＳＡ及Ｗｅｓｔｅｒｎ ｂｌｏｔ结果表明，构建的两株重组病毒分别表达了ＨＩＶ－１Ｇａｐ ｐ２４及ｐ１７－ｐ２４融合蛋白。电镜观察证实，Ｇａｇ ｐ２４及ｐ１７－２４重组蛋白均可形成病毒样粒子。重组病毒可诱导小鼠产生抗ＨＩＶ－１Ｇａｐ ｐ２４抗体。重组病毒感染ＢＨＫ２１细胞后，可见由 |
| 关 键 词： | HIV－1 痘苗病毒 核心蛋白 表达 性质 疫苗 |
| 文章编号： | 1000-3061(2000)01-0065-04 |
| 修稿时间： | 1998-10-08 |
Expression and Characterization of HIV-1 Gag p17-p24 Protein |
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| LI Ti-yuan,JIN Ning-yi,WANG Hong-wei,GUO Zhi-ru,FANG Hou-hua,AN Ru-guo,YIN Zhen. Expression and Characterization of HIV-1 Gag p17-p24 Protein[J]. Chinese journal of biotechnology, 2000, 16(1): 65-68 | |
| Authors: | LI Ti-yuan JIN Ning-yi WANG Hong-wei GUO Zhi-ru FANG Hou-hua AN Ru-guo YIN Zhen |
| Affiliation: | LI Ti-yuan;(Norman Bethune University of Medical Sciences, Changchun 130021);JIN Ning-yi,WANG Hong-wei,GUO Zhi-ru,FANG Hou-hua;(Changchun University of Agriculture and Animal Scitences, Changchun 130062);AN Ru-guo;(Norman Bethune University of Medical Sciences, Changchun 130021) |
| Abstract: | The biological and immunological characteristics of HIV-1 core protein p17-24 expressed by recombinant vac cinia viruses were studied. The results of indirect immunofluorescence assay (I-IFA), Westem blot and dot ELISA showed that the two recombinants could express the p24 gag and p17 24 gag fusion proteins in infected cell lines respectively. The electromicroscope observation revealed that the expressed proteins could also assemble into virus-like particles. The recom binant vaccinia virases can also stimulate mice for the formation of anti HIV-1 Gag p24 antibody. When infected with the recombinant virtuses, the chromosome DNA ladder caused by the apoptosis of the BHK cell was observed. |
| Keywords: | HIV-1 Gag vaccinia virus |
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