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In situ proteolysis for protein crystallization and structure determination |
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| Authors: | Dong Aiping Xu Xiaohui Edwards Aled M;Midwest Center for Structural Genomics;Structural Genomics Consortium Chang Changsoo Chruszcz Maksymilian Cuff Marianne Cymborowski Marcin Di Leo Rosa Egorova Olga Evdokimova Elena Filippova Ekaterina Gu Jun Guthrie Jennifer Ignatchenko Alexandr Joachimiak Andrzej Klostermann Natalie Kim Youngchang Korniyenko Yuri Minor Wladek Que Qiuni Savchenko Alexei Skarina Tatiana Tan Kemin Yakunin Alexander Yee Adelinda Yim Veronica Zhang Rongguang Zheng Hong Akutsu Masato Arrowsmith Cheryl Avvakumov George V Bochkarev Alexey Dahlgren Lars-Göran Dhe-Paganon Sirano |
| Institution: | Structural Genomics Consortium, University of Toronto, 100 College Street, Toronto, Ontario M5G 1L5, Canada. |
| Abstract: | We tested the general applicability of in situ proteolysis to form protein crystals suitable for structure determination by adding a protease (chymotrypsin or trypsin) digestion step to crystallization trials of 55 bacterial and 14 human proteins that had proven recalcitrant to our best efforts at crystallization or structure determination. This is a work in progress; so far we determined structures of 9 bacterial proteins and the human aminoimidazole ribonucleotide synthetase (AIRS) domain. |
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