Establishment and characterization of a spontaneously immortalized mouse ameloblast-lineage cell line |
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Authors: | Nakata Akira Kameda Takashi Nagai Hirokazu Ikegami Kenji Duan Yuqing Terada Kunihiko Sugiyama Toshihiro |
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Affiliation: | Department of Biochemistry, Akita University School of Medicine, Hondo 1-1-1, Akita 010-8543, Japan. |
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Abstract: | Tooth development was cooperatively regulated by the epithelial ameloblasts and mesenchymal odontoblasts. Ameloblasts secrete enamel matrix, critical for enamel formation. While there are several reports about establishment of immortalized ameloblast-like cells by introducing viral oncogene, we tried to establish a spontaneously immortalized ameloblast-lineage cell line, maintaining the cell type specific character, including the ability to induce in vitro bio-mineralization. The established cell line (ameloblast-lineage cell; ALC) maintained the expression of several ameloblast specific genes (Amelogenin, Tuftelin, and Enamelin) in long-term culture. They formed calcified nodules after the induction by medium switching from SMEM to DMEM, having high-level alkaline-phosphatase activity. The size and number of calcified nodule formation were enhanced by TGF-beta treatment. Six weeks after sub-cutaneous implantation of ALC to athymic nude mice, we ectopically observed enamel epithelium like structure formation, chondrogenesis, and calcification. These data indicate that ALC is a useful experimental tool to analyze ameloblast character. |
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Keywords: | Ameloblast Spontaneous immortalization Enamel matrix Calcification |
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