Lipase production by diverse phylogenetic clades of Aureobasidium pullulans |
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| Authors: | Timothy D. Leathers Joseph O. Rich Amber M. Anderson Pennapa Manitchotpisit |
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| Affiliation: | 1. Renewable Product Technology Research Unit National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, 1815 North University Street, Peoria, IL, 61604, USA
2. Biochemistry Unit, Department of Medical Sciences, Faculty of Science, Rangsit University, 52/347 Muang Ake, Phaholyothin Road, Lakhok, Pathumthani, 12000, Thailand
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| Abstract: | Thirty-nine strains representing 12 diverse phylogenetic clades of Aureobasidium pullulans were surveyed for lipase production using a quantitative assay. Strains in clades 4 and 10 produced 0.2–0.3 U lipase/ml, while color variant strain NRRL Y-2311-1 in clade 8 produced 0.54 U lipase/ml. Strains in clade 9, which exhibit a dark olivaceous pigment, produced the highest levels of lipase, with strain NRRL 62034 yielding 0.57 U lipase/ml. By comparison, Candida cylindracea strain NRRL Y-17506 produced 0.05 U lipase/ml under identical conditions. A. pullulans strain NRRL 62034 reached maximal lipase levels in 5 days on lipase induction medium, while A. pullulans strain NRRL Y-2311-1 and strains in clades 4 and 10 were highest after 6 days. A. pullulans strain NRRL Y-2311-1 and strains in clade 9 produced two extracellular proteins in common, at >50 and <37 kDa. |
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