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| PRRSV-GSLZ-1/2009细胞毒和组织毒致病特征的比较 | |
| 引用本文： | 杜平,尚佑军,刘学荣,董文教,贺延玉,黄银君,牟克斌. PRRSV-GSLZ-1/2009细胞毒和组织毒致病特征的比较[J]. 微生物学通报, 2011, 38(1): 63-69 |
| 作者姓名： | 杜平 尚佑军 刘学荣 董文教 贺延玉 黄银君 牟克斌 |
| 作者单位： | 1. 中农威特生物科技股份有限公司,甘肃,兰州,730046;中国农业科学院兰州兽医研究所,家畜疫病病原生物学国家重点实验室,农业部畜禽病毒学重点实验室国家口蹄疫参考实验室,甘肃,兰州,730046 2. 中国农业科学院兰州兽医研究所,家畜疫病病原生物学国家重点实验室,农业部畜禽病毒学重点实验室国家口蹄疫参考实验室,甘肃,兰州,730046 3. 中农威特生物科技股份有限公司,甘肃,兰州,730046 4. 甘肃农业大学研究测试中心,甘肃,兰州,730070 |
| 基金项目： | 国家863计划项目(No. 2006AA10A204) |
| 摘 要： | 为了研究猪繁殖与呼吸综合征病毒(Porcine reproductive and respiratory syndrome virus,PRRSV)野毒株在细胞适应中的遗传变异与其致病性的关系。在进行遗传变异分析后,将GSLZ-1/2009细胞适应毒和组织毒同时接种36日龄健康仔猪,对感染后的临床症状、体征变化、抗体及炎性因子水平进行了实时监测和动力学分析,并对组织病变进行了显微观察。结果显示:在细胞适应过程中致病相关基因并未发生任何突变,但细胞适应株所诱发的抗体及炎症因子反应均明显较组织毒弱,且所造成的病理损伤也比较轻微。表明PRRSV野毒株在细胞适应过程中毒力会减弱,而毒株的弱化可能由遗传变异之外的因素决定。 |
| 关 键 词： | 猪繁殖与呼吸综合征病毒 病毒特性 致病特征 |
| 收稿时间： | 2010-07-23 |
| 修稿时间： | 2010-11-03 |
Comparison of pathogenic characteristics between PRRSV-GSLZ-1/2009 cell propagated virus and field sample virus |
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| DU Ping,SHANG You-Jun,LIU Xue-Rong,DONG Wen-Jiao,HE Yan-Yu,HUANG Yin-Jun and MU Ke-Bin. Comparison of pathogenic characteristics between PRRSV-GSLZ-1/2009 cell propagated virus and field sample virus[J]. Microbiology China, 2011, 38(1): 63-69 | |
| Authors: | DU Ping SHANG You-Jun LIU Xue-Rong DONG Wen-Jiao HE Yan-Yu HUANG Yin-Jun MU Ke-Bin |
| Affiliation: | China Agriculture Veterinary Biology Science and Technology Corporation, Lanzhou, Gansu 730046, China; Key Laboratory of Animal Virology, Ministry of Agriculture; State Key Laboratory of Veterinary Etiological Biology; National Foot-and-Mouth Disease Viru;Key Laboratory of Animal Virology, Ministry of Agriculture; State Key Laboratory of Veterinary Etiological Biology; National Foot-and-Mouth Disease Virus Referenced Laboratory; Lanzhou Veterinary Research Institute, Chinese Academy of Agriculture Science,;China Agriculture Veterinary Biology Science and Technology Corporation, Lanzhou, Gansu 730046, China;China Agriculture Veterinary Biology Science and Technology Corporation, Lanzhou, Gansu 730046, China;Instrumental Research & Analysis Center of Gansu Agricultural University, Lanzhou, Gansu 730070, China;China Agriculture Veterinary Biology Science and Technology Corporation, Lanzhou, Gansu 730046, China;China Agriculture Veterinary Biology Science and Technology Corporation, Lanzhou, Gansu 730046, China |
| Abstract: | The objective of this study was to analyze the correlation between genetic variation and pathogenicity of field sample virus during the propagation in sensitive cells. 36-day-old pigs were challenged with PRRSV GSLZ-1/2009 cell propagated virus and field sample virus after genetic variation analyse. Carry out real-time monitor and dynamics analyse to clinical symptoms, signs of change and the levels of antibody and inflammatory factors, and the histopathologic changes were observed under light microscope. The results showed that the disease-related genes was no variation, the antibody, inflammatory factors response and histopathologic lesions induced by field sample virus were more powerful than those induced by cell propagated virus. The researches indicated that virulence of field PRRSV could be weakened when it propagated in cells, and there are other possible factors except genetic variation playing on the role of virulence weaken. |
| Keywords: | Porcine reproductive and respiratory syndrome virus Virus characters Pathogenic char-acteristics |
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