Culture and characterization of bovine mesenteric lymphatic endothelium |
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Authors: | B. E. Jones L. C. J. Yong |
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Affiliation: | (1) School of Pathology, University of New South Wales, Kensington, P.O. Box 1, 2033 Sydney, Australia |
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Abstract: | Summary Lymphatic endothelial cells isolated from bovine mesenteric lymphatic vessels were cultured and characterized. Lymphatic endothelial cells grew as a monolayer displaying an elongated morphology in preconfluent primary cultures. When confluent, the cells exhibited a polygonal morphology to form a “cobblestone” pattern previously described for cultured vascular endothelium. All culture lymphatic endothelial cells expressed Factor VIII-related antigen and boundUlex europaeus I lectin. Ultrastructurally, cultured lymphatic endothelium was characterized by the presence of Weibel-Palade bodies as well as the usual cytoplasmic organelles. |
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Keywords: | lymphatic endothelium Factor VIII-related antigen Weibel-Palade body tissue culture |
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