Application of silver stains to gastric endocrine cells in plastic sections |
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Authors: | Edward I. Weinshelbaum Jan M. Pittman |
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Affiliation: | (1) Department of Surgery, Veterans Administration Hospital, Lake City, Florida;(2) The Veterans Administration Hospital, Gainesville, Florida |
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Abstract: | Summary A simultaneous light and electron microscopic study of mouse gastric mucosa was made to determine whether the silver nitrate methenamine stain of Duk-Ho Lee could be used to stain gastric endocrine-like cells in plastic embedded tissue. Examination of consecutive thick and thin sections showed that this stain blackened the granules of the predominant type of endocrine-like cell present. Blackening of the granules with silver occured in tissue fixed in osmium tetroxide solution with or without dichromate salt or in tissue fixed in glutaraldehyde then treated with osmium. The intensity of staining was deepest in the osmium-dichromate fixed tissue, but the glutaraldehyde-osmium procedure gave less interference from diffuse silver impregnation and better preservation of detail for electron microscopy. |
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