Characterization of Temperate <Emphasis Type="Italic">Lactobacillus gasseri</Emphasis> Phage LgaI and Its Impact as Prophage on Autolysis of Its Lysogenic Host Strains |
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Authors: | Elsayed A Ismail Horst Neve Arnold Geis Knut J Heller |
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Institution: | (1) Department of Microbiology and Biotechnology, Max Rubner-Institut, Federal Institute of Nutrition and Food, Hermann-Weigmann-Str. 1, P.O. Box 6069, Kiel, 24121, Germany;(2) Present address: Food Science Department, Faculty of Agriculture at Moshtohor, Benha University, Moshtohor, Toukh, Qaluibyia, Benha, Egypt |
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Abstract: | We show by electron microscopy that Lactobacillus gasseri phage LgaI, a temperate phage residing in the chromosome of Lactobacillus gasseri ATCC33323, belongs to the family of Myoviridae phages. The LgaI DNA is packed by the “head-full” mechanism, as demonstrated by analysis of restriction patterns of heated
(74°C) or non-heated DNA. By isolating prophage-cured cells, we were able to demonstrate phage LgaI to be responsible for
the strong autolytic phenotype observed for Lactobacillus gasseri ATCC33323. In addition, we show that a copy of the LgaI prophage resides in the chromosome of Lactobacillus gasseri NCK102. The LgaI prophage was not inducible in L. gasseri NCK102-adh by mitomycin C, however, it apparently contributed to the autolytic phenotype of this strain. |
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