极低密度脂蛋白对巨噬细胞载脂蛋白E基因表达的调节

巨噬细胞（M）是动脉粥样硬化（AS）斑块中泡沫细胞的主要来源之一，它可通过其表面的多种脂蛋白受体介导摄取合APOB或APOE的脂蛋白，导致M的脂质增加．此外，M有较强的分泌ApOE的能力1]，这一现象虽已发现多年，但意义尚不清楚VLDL是一种含APOE的脂蛋白，它能通过M膜上识别APOE的受体进入M，从而促进Mg内脂质堆积，并与AS密切相关2,3]．流行病学调查发现在我国的高脂血症中2／3为VLDL升高为主的高甘油三酯（HTG）血症．另外，Evans等报道HTG血症患者的血浆中以ApoE－poorVLDL升高为主，它们在血浆中清除的速率较Apo…

Regulation of ApoE Gene Expression in Mouse Peritoneal Macrophages by Very Low Density Lipoproteins

Normal human very low density lipoprotein (VLDL) can be separated into two subfractions on Heparin-Sepharose 6B chromatography. one is called ApoE-poor VLDL and the other, ApoE-rich VLDL. They are different in compositions. Mouse peritoneal macrophages (MPM)were incubated with ApoE-poor VLDL or ApoE-rich VLDL at same concentrations for 24 hours.Increases of ApoE mRNA contents in both groups were higher than that in control and the highestApoE mRNA content was seen in MPM incubated with ApoE-poor VLDL. The results suggestthat VLDL can stimulate ApoEgene expression in MPM and the ApoE-poor VLDL exhibits morepronounced effect. It was predicted that the ApoE secreted by MPM might incorporate intoVLDL, especially the ApoE-poor VLDL, and enhance the uptake of these lipoproteins by MPM orother local cells via ApoE mediated receptor pathways.