Therapeutic potential of BAK gene silencing in aluminum induced neural cell degeneration |
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Authors: | QL Zhang Q Niu YT Shi PY Niu CY Liu L Zhang C Zhang |
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Institution: | aShanxi Medical University, Taiyuan, Shanxi 030001, China;bShanxi Provincial Cancer Hospital, Taiyuan, Shanxi 030001, China;cSchool of Public Health and Family Medicine, Capital Medical University, Beijing100069, China |
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Abstract: | Previous studies have demonstrated robust BAK gene silencing via RNA interference (RNAi). To investigate whether BAK RNAi may serve as a co-therapeutic agent in neural cell death, we herein established a cell degeneration model using a human neuroblastoma cell line (SH-SY5Y) treated by aluminum (Al). Combining cell viability assays and expression analyses by QRT (quantitative real-time)-PCR and immunocytochemistry, we selected and validated the optimal small interfering RNA (siRNA) from three candidate siRNAs for the BAK gene. Our data identified siRNA1 as the most effective siRNA; the optimal concentration of the transfection agent was 10 nM and the optimal incubation period was 24 h. The transfection and knockdown efficiency was 93% and 58%, respectively, which closely correlated with the BAK protein expression. SH-SY5Y cells with BAK knockdown showed a clear resistance against cell death and Al-induced apoptosis. These results indicate that genetic inactivation of BAK could be an effective strategy in delaying the onset of apoptosis in Al-treated cells, and exemplify the therapeutic potential of RNAi-based methods for the treatment of neural cell degeneration. |
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Keywords: | BAK Apoptosis siRNA RNA interference Aluminum |
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