Effect of DNA methylation on protein-DNA interaction of HL-60 cells

HL-60 cells have been induced with differentiation index 16% by S-adenosyl-L-methionine (SAM) as inducer in the presence of optimum concentration of 10 pmol/L. The methylation level of genome DNA determined by HPLC is increased during cell differentiation. When restriction endonucleaseHae III,Sma I,Sal I,XhoI andHind III which are sensitive to 5-methyicytoside were used to cleave the genome DNA, a resistance effect was found. The interaction between DNA and DNA binding proteins is changed by using gel retarding test.