Metabolism of d-xylose in Schizosaccharomyces pombe cloned with a xylose isomerase gene |
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Authors: | Err-Cheng Chan Peter Pear Ueng Li Fu Chen |
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Affiliation: | (1) Department of Food Science, Purdue University, 47907 West Lafayette, IN, USA;(2) Department of Botany and Plant Pathology, Purdue University, 47907 West Lafayette, IN, USA |
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Abstract: | Summary The Escherichia coli xylose isomerase gene was transformed into Schizosaccharomyces pombe for direct d-xylose utilization. In order to understand d-xylose metabolism and determine the limiting factors on d-xylose utilization by the transformed yeast, d-xylose transport, xylose isomerization, and xylulose phosphorylation were investigated. The results indicated that low activity of xylose isomerization in the cloned yeast was the limiting step for d-xylose fermentation. An in vitro study showed that yeast proteases decreased xylose isomerase activity. Xylitol, a by-product of d-xylose fermentation, had no effect on the activity of xylose isomerase. |
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