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Stoichiometry of phosphate loss from sea urchin sperm guanylate cyclase during fertilization
Authors:V D Vacquier  G W Moy
Affiliation:1. Department of Molecular Sensory Systems, Center of Advanced European Studies and Research (caesar), Bonn, Germany;2. Center of Reproductive Medicine and Andrology, University Hospital Münster, University of Münster, Münster, Germany;3. Department of Chemistry, University of California, Berkeley, CA, United States;4. Department of Molecular & Cell Biology, University of California, Berkeley, CA, United States;5. Helen Wills Neuroscience Institute, University of California, Berkeley, CA, United States
Abstract:Spermatozoa of the sea urchin Arbacia punctulata possess a phosphorylated guanylate cyclase as a major glycoprotein of the flagellar plasma membrane. When sperm cells contact the jelly layer surrounding the egg, the peptide "resact" binds the sperm cell surface and triggers the dephosphorylation of the cyclase. A large decrease in cyclase activity accompanies dephosphorylation. Before treatment of sperm cells with egg jelly the enzyme contains 17.95 +/- 1.24 moles phosphate per mole cyclase. After treatment of sperm cells with egg jelly this number decreases to 2.57 +/- 0.42. Based on a molecular weight of 137,250 for the peptide chain, approximately 15 phosphate groups are lost per molecule of guanylate cyclase at fertilization.
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