UVB radiation induced effects on cells studied by FTIR spectroscopy |
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Authors: | Lucia Di Giambattista P Grimaldi S Gaudenzi D Pozzi M Grandi S Morrone I Silvestri A Congiu Castellano |
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Affiliation: | 1. Dipartimento di Fisica, Università di Roma Sapienza, Piaz.le A. Moro, 2, 00185, Rome, Italy 2. CISB (Interdepartmental Research Centre for Biomedical Systems Models and Information Analysis), Rome, Italy 3. Dipartimento di Medicina Sperimentale, Università di Roma Sapienza, V.le Regina Elena, 324, 00161, Rome, Italy 4. Poliambulatorio La Torre, V. M. Ponzio,10, 10141, Torino, Italy
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Abstract: | We have made a preliminary analysis of the results about the effects on tumoral cell line (lymphoid T cell line Jurkat) induced
by UVB radiation (dose of 310 mJ/cm2) with and without a vegetable mixture. In the present study, we have used two techniques: Fourier transform infrared spectroscopy
(FTIR) and flow cytometry. FTIR spectroscopy has the potential to provide the identification of the vibrational modes of some
of the major compounds (lipid, proteins and nucleic acids) without being invasive in the biomaterials. The second technique
has allowed us to perform measurements of cytotoxicity and to assess the percentage of apoptosis. We already studied the induction
of apoptotic process in the same cell line by UVB radiation; in particular, we looked for correspondences and correlations
between FTIR spetroscopy and flow cytometry data finding three highly probable spectroscopic markers of apoptosis (Pozzi et
al. in Radiat Res 168:698–705, 2007). In the present work, the results have shown significant changes in the absorbance and
spectral pattern in the wavenumber protein and nucleic acids regions after the treatments. |
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