| 葡萄子房胚性细胞悬浮系的建立及其生长特性研究 |
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| 引用本文： | 郭殿京 王东凯. 葡萄子房胚性细胞悬浮系的建立及其生长特性研究[J]. 生物技术, 1995, 5(2): 16-18 |
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| 作者姓名： | 郭殿京 王东凯 |
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| 作者单位： | 东北农业大学园艺系 |
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| 摘 要： | 由葡萄栽培品种“蜜汁”子房诱导的愈伤组织，经不同浓度２，４－Ｄ试验表明，０．５ｍｇ／Ｌ的２，４－Ｄ最有利于形成胚性细胞；该浓度下形成的质地紧密适度的愈伤组织经２个月的悬浮振荡培养，成功地建立了分散性好、生长旺盛的胚性细胞悬浮系；并通过培养过程中生长曲线的测定，确定了细胞悬浮系的生长特性。
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| 关 键 词： | 葡萄 子房 胚性细胞悬浮系 |
Establishment and Characterization of Grape Embryogenic Cell Suspension Culture |
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| Guo Dianjing, Wang Dongkai, Zhu Yanming. Establishment and Characterization of Grape Embryogenic Cell Suspension Culture[J]. Biotechnology, 1995, 5(2): 16-18 |
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| Authors: | Guo Dianjing Wang Dongkai Zhu Yanming |
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| Abstract: | Ovary--derived calti were induced from grape cultivar H Mizhin on B, medium suppemented with 0. sing/L 2,4--D, 2. 0mg/L BA, 20g/L sucrose and 0. 6% agar. Friable embryogenic calli were selecte as starting material for establishment of cell suspension. Finely--dispersed, fast--growing and highly--embryogenic cell suspension were achieved after 2months of subculture. Growth characteristics were determined by establishment of cellgrowth curve. |
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| Keywords: | Grape: Ovary: Embry genie cell suspension |
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