|
|||||
|
|
Comparison of Expression of Inflammatory Cytokines in the Spinal Cord Between Young Adult and Aged Beagle Dogs |
|
| Authors: | Dae Hwan Lee Ji Hyeon Ahn Joon Ha Park Bing Chun Yan Jeong-Hwi Cho In Hye Kim Jae-Chul Lee Sang-Hun Jang Myoung Hyo Lee In Koo Hwang Seung Myung Moon Bonghee Lee Jun Hwi Cho Hyung-Cheul Shin Jin Sang Kim Moo-Ho Won |
| Institution: | 1. Laboratory of Neuroscience, Department of Physical Therapy, College of Rehabilitation Science, Daegu University, Gyeongsan, 712-714, South Korea 2. Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon, 200-701, South Korea 3. Department of Physical Therapy, Gimcheon University, Gimcheon, 740-704, South Korea 4. Department of Anatomy and Cell Biology, College of Veterinary Medicine, and Research Institute for Veterinary Science, Seoul National University, Seoul, 151-742, South Korea 5. Department of Neurosurgery, Dongtan Sacred Heart Hospital, College of Medicine, Hallym University, Hwaseong, 445-170, South Korea 6. Center for Genomics and Proteomics, Lee Gil Ya Cancer and Diabetes Institute, Gachon University of Medicine and Science, Incheon, 406-840, South Korea 7. Department of Emergency Medicine, School of Medicine, Kangwon National University, Chuncheon, 200-701, South Korea 8. Institute of Medical Sciences, Kangwon National University Hospital, School of Medicine, Kangwon National University, Chuncheon, 200-701, South Korea 9. Department of Physiology, College of Medicine, Hallym University, Chuncheon, 200-702, South Korea |
| Abstract: | Aging is an inevitable process that occurs in the whole body system accompanying with many functional and morphological changes. Inflammation is known as one of age-related factors, and inflammatory changes could enhance mortality risk. In this study, we compared immunoreactivities of inflammatory cytokines, such as interleukin (IL)-2 (a pro-inflammatory cytokine), its receptor (IL-2R), IL-4 (an anti-inflammatory cytokine), and its receptor (IL-4R) in the cervical and lumbar spinal cord of young adult (2–3 years old) and aged (10–12 years old) beagle dogs using immunohistochemistry and western blotting. IL-2 and IL-2R-immunoreactive nerve cells were found throughout the gray matter of the cervical and lumbar spinal cord of young adult and aged dogs. In the spinal cord neurons of the aged dog, immunoreactivity and protein levels were apparently increased compared with those in the young adult dog. Change patterns of IL-4- and IL-4R-immunoreactive cells and their protein levels were also similar to those in IL-2 and IL-2R; however, IL-4 and IL-4R immunoreactivity in the periphery of the neuronal cytoplasm in the aged dog was much stronger than that in the young adult dog. These results indicate that the increase of inflammatory cytokines and their receptors in the aged spinal cord might be related to maintaining a balance of inflammatory reaction in the spinal cord during normal aging. |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ | |