功能歧化分析揭示代谢型谷氨酸受体亚家族VFTM的分化决定位点

目的：研究哺乳动物代谢型谷氨酸受体（mGluR）亚家族捕蝇夹结构模块（VFTM）的系统进化形式,确定其VFTM的功能分化决定位点。方法：通过基于系统进化的功能歧化分析,根据贝叶斯模型计算VFTM的选择性限制位点,推测功能分化决定位点。结果：mGluR亚家族VFTM的祖先通过2次基因复制,在不同的氨基酸位点进行选择性限制,形成3组不同的mGluR亚家族成员。结论：不同功能分化决定位点的选择性功能歧化塑造了mGluR亚家族VFTM的结构与功能,为进一步研究mGluR亚家族的激活机制奠定了基础。

Functional Divergence Analysis Reveals the Determinant Sites of the VFTM of Metabotropic Glutamate Receptor Subfamily

Objective： To investigate the evolutionary pattern of Venus flytrap module（VFTM） of metabotropic glutamate receptor（mGluR） subfamily and validate the determinant sites involved functional divergence. Methods： The＇ functional divergence analysis based on the evolutionary pattern of VFTM was used to identify the determinant sites involved functional divergence according to the Bayesian post probability model. Results： The constraints on different sites were resulted in the three groups of mGluR via two continuous gene duplication events. Conclusion： The selective functional constraints on the determinant sites shaped the structures and functions of the VFTM of mGluR subfamily, which shed light on the activation mechanism of mGluR subfamily.