A restriction endonuclease map of Streptomyces phage VWB |
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| Authors: | Jozef Anné , Patrick Verheyen, Guido Volckaert Hendrik Eyssen |
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| Affiliation: | (1) Rega Institute, University of Leuven, Minderbroedersstraat 10, B-3000 Leuven, Belgium |
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| Abstract: | Summary A physical map of the actinophage VWB has been constructed using the restriction endonucleases BglII, ClaI, EcoRI, EcoRV, HindIII, KpnI and SphI. Phage VWB, genome size 47.3 kb, propagates on Streptomyces venezuelae, and it can also lysogenise this species. The three BglII-generated fragments of VWB DNA were cloned in pBR322, and subsequently mapped. In this manner the restriction map of the VWB phage genome was constructed.Abbreviations dam DNA adenine methylase activity - kb kilobase pairs - :: novel joint |
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