A Multi‐Omics Analysis of Glycine max Leaves Reveals Alteration in Flavonoid and Isoflavonoid Metabolism Upon Ethylene and Abscisic Acid Treatment |
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| Authors: | Ravi Gupta Cheol Woo Min Katharina Kramer Ganesh Kumar Agrawal Randeep Rakwal Ki‐Hun Park Yiming Wang Iris Finkemeier Sun Tae Kim |
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| Institution: | 1. Department of Plant Bioscience, Life and Industry Convergence Research Institute, Pusan National University, Miryang, Republic of Korea;2. Plant Proteomics Group, Max Planck Institute for Plant Breeding Research, Cologne, Germany;3. Research Laboratory for Biotechnology and Biochemistry, Kathmandu, Nepal;4. GRADE Academy Private Limited, Birgunj, Nepal;5. Faculty of Health and Sport Sciences, University of Tsukuba, Tsukuba, Japan;6. Global Research Center for Innovative Life Science, Peptide Drug Innovation, School of Pharmacy and Pharmaceutical Sciences, Hoshi University, Tokyo, Japan;7. Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju, Republic of Korea;8. Department of Plant Microbe Interactions, Max Planck Institute for Plant Breeding Research, Cologne, Germany;9. Institute of Plant Biology and Biotechnology, University of Muenster, Muenster, Germany |
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| Abstract: | Phytohormones are central to plant growth and development. Despite the advancement in our knowledge of hormone signaling, downstream targets, and their interactions upon hormones action remain largely fragmented, especially at the protein and metabolite levels. With an aim to get new insight into the effects of two hormones, ethylene (ET) and abscisic acid (ABA), this study utilizes an integrated proteomics and metabolomics approach to investigate their individual and combined (ABA+ET) signaling in soybean leaves. Targeting low‐abundance proteins, our previously established protamine sulfate precipitation method was applied, followed by label‐free quantification of identified proteins. A total of 4129 unique protein groups including 1083 differentially modulated in one (individual) or other (combined) treatments were discerned. Functional annotation of the identified proteins showed an increased abundance of proteins related to the flavonoid and isoflavonoid biosynthesis and MAPK signaling pathway in response to ET treatment. HPLC analysis showed an accumulation of isoflavones (genistin, daidzein, and genistein) upon ET treatment, in agreement with the proteomics results. A metabolome analysis assigned 79 metabolites and further confirmed the accumulation of flavonoids and isoflavonoids in response to ET. A potential cross‐talk between ET and MAPK signaling, leading to the accumulation of flavonoids and isoflavonoids in soybean leaves is suggested. |
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| Keywords: | ABA ethylene flavonoids isoflavonoids metabolomics proteomics |
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