Antibody Profiling by Proteome Microarray with Multiplex Isotype Detection Reveals Overlap between Human and Aotus nancymaae Controlled Malaria Infections |
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| Authors: | Omid Taghavian Aarti Jain Chester J Joyner Sunny Ketchum Rie Nakajima Algis Jasinskas Li Liang Rich Fong Christopher King Bryan Greenhouse Maxwell Murphy Jason Bailey Mary R Galinski John W Barnwell Christopher V Plowe D Huw Davies Philip L Felgner |
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| Institution: | 1. Department of Medicine, Division of Infectious Diseases, University of California Irvine, Irvine, CA, USA;2. International Center for Malaria Research, Education and Development, Emory Vaccine Center, Yerkes National Primate Research Center, Emory University, Atlanta, GA, USA;3. Grace Bio‐Labs Inc., Bend, OR, USA;4. Center for Global Health and Diseases, Case Western Reserve University, Cleveland, OH, USA;5. Department of Medicine, University of California, San Francisco, CA, USA;6. Division of Infectious Diseases, Department of Medicine, Emory University, Atlanta, GA, USA;7. Centers for Disease Control and Prevention, Atlanta, GA, USA;8. Howard Hughes Medical Institute/Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, MD, USA |
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| Abstract: | The development of vaccines against malaria and serodiagnostic tests for detecting recent exposure requires tools for antigen discovery and suitable animal models. The protein microarray is a high‐throughput, sample sparing technique, with applications in infectious disease research, clinical diagnostics, epidemiology, and vaccine development. We recently demonstrated Qdot‐based indirect immunofluorescence together with portable optical imager ArrayCAM using single isotype detection could replicate data using the conventional laser confocal scanner system. We developed a multiplexing protocol for simultaneous detection of IgG, IgA, and IgM and compared samples from a controlled human malaria infection model with those from controlled malaria infections of Aotus nancymaae, a widely used non‐human primate model of human malaria. IgG profiles showed the highest concordance in number of reactive antigens; thus, of the 139 antigens recognized by human IgG antibody, 111 were also recognized by Aotus monkeys. Interestingly, IgA profiles were largely non‐overlapping. Finally, on the path toward wider deployment of the portable platform, we show excellent correlations between array data obtained in five independent laboratories around the United States using the multiplexing protocol (R2: 0.60–0.92). This study supports the use of this platform for wider deployment, particularly in endemic areas where such a tool will have the greatest impact on global human health. |
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| Keywords: | antibody isotype malaria multiplex protein microarray quantum dots |
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