In vitro plant regeneration from callus derived from root explants of Lathyrus sativus |
| |
| Authors: | P K Roy G K Barat S L Mehta |
| |
| Institution: | (1) Division of Biochemistry, Indian Agricultural Research Institute, 110 012 New Delhi, India |
| |
| Abstract: | Protocols have been developed for the in vitro production of plants from callus derived from root explants of Lathyrus sativus cv. P-24. Callus and shoot regeneration were achieved only in MS medium supplemented with 10.7  M naphthaleneacetic acid and an increased concentration of kinetin (0.9 M for 14 days to 1.4 M for 18 days) during callusing. The shoots obtained rooted in 1/2 MS supplemented with 0.5 M indolebutyric acid. During the year plants have been regenerated several times. The requirement for growth regulators is very specific and narrow.Abbreviations IAA
indoleacetic acid
- IBA
indolebutyric acid
- NAA
naphthaleneacetic acid
- BA
benzyladenine |
| |
| Keywords: | grain legume grass pea rooting tissue culture |
| 本文献已被 SpringerLink 等数据库收录！ |
