Sequencing, cloning, and high-level expression of the pfp gene, encoding a PP(i)-dependent phosphofructokinase from the extremely thermophilic eubacterium Dictyoglomus thermophilum |
| |
Authors: | Ding Y H Ronimus R S Morgan H W |
| |
Affiliation: | Thermophile Research Unit, Department of Biological Sciences, The University of Waikato, Hamilton, New Zealand. yrd6@hotmail.com |
| |
Abstract: | The sequencing, cloning, and expression of the pfp gene from Dictyoglomus thermophilum, which consists of 1,041 bp and encodes a pyrophosphate-dependent phosphofructokinase, are described. A phylogenetic analysis indicates that the enzyme is closely related to the pyrophosphate-dependent enzyme from Thermoproteus tenax. The recombinant and native enzymes share a high degree of similarity for most properties examined. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|