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Monoribosomal attachment to messenger ribonucleic acid in sodium fluoride-treated rabbit reticulocytes (Short Communication)
Authors:Michael Geraghty   Marilyn Galler   Fred Schiffman     Michael Freedman
Affiliation:Department of Medicine, New York University School of Medicine, 550 First Avenue, New York, N.Y. 10016, U.S.A.
Abstract:Mild proteolysis with Pronase selectively dissociates ribosomes not attached to mRNA into subunits; ribosomes attached to mRNA remain intact. A portion of monoribosomes from reticulocytes incubated with NaF resisted proteolytic dissociation. Recovery of mRNA from monoribosomes of NaF-treated reticulocytes therefore may be explained by persistent attachment of some monoribosomes to mRNA.
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