Enzymic synthesis of saccharopine- 14 C |
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| Authors: | A K Sinha |
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| Affiliation: | 1. Renewable Energy Unit, CICY, Parque Científico y Tecnológico de Yucatán, Way to Sierra Papacal-Chuburná Puerto, Km 5, Sierra Papacal, Mérida, Yucatán 97302, Mexico;2. Lactic Acid Bacteria Research Group, Faculty of Engineering and Administration, Universidad Nacional de Colombia Campus Palmira, Palmira, Valle del Cauca, Colombia;1. College of Agriculture, Central Agricultural University, Imphal, Manipur, 795004, India;2. Department of Biotechnology, GST, Gandhi Institute of Technology and Management (GITAM), Visakhapatnam 530045, A. P., India;3. Department of Forestry, Mizoram University, Aizawl 796004, India;4. Center for Energy and Environment, Malaviya National Institute of Technology Jaipur, Rajasthan 302017, India |
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| Abstract: | A simple enzymic method for the preparation of radioactive saccharopine has been described using l-lysine-UL-14C, α-ketoglutaric acid, NADH + H+, and a yeast cell-free extract. Radioactive saccharopine was purified by chromatography on Dowex formate and crystallized from ethanol. |
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