| 抑制Rac1蛋白活化阻碍胚胎发育早期血管新生 |
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| 引用本文： | 张剑,韩雅玲,康建,张效林,齐岩梅,闫承慧,李少华. 抑制Rac1蛋白活化阻碍胚胎发育早期血管新生[J]. 生物化学与生物物理进展, 2009, 36(6): 696-701. DOI: 10.3724/SP.J.1206.2008.00685 |
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| 作者姓名： | 张剑 韩雅玲 康建 张效林 齐岩梅 闫承慧 李少华 |
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| 作者单位： | 1. 沈阳军区总医院全军心血管病研究所心血管内科,沈阳,110016
2. Department of Pathology and Laboratory Medicine,Robert Wood Johnson Medical School,New Jersey 08854,USA |
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| 基金项目： | 国家自然科学基金资助项目(面上课题30770793, 30800465) |
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| 摘 要： | 小G蛋白Rac1在胚胎发育早期血管形成尤其是内皮发生过程中的作用尚不清楚．采用胚胎干细胞(ESCs)为模型，建立稳定表达持续表达型Rac1(G12V)和显性失活型Rac1(T17N)编码序列的小鼠ESCs并制备胚胎小体(EBs)，诱导分化后观察Rac1(G12V)和Rac1(T17N)对内皮细胞分化和迁移功能的影响．采用相差显微镜观察EBs发育和分化特征，Pull down分析Rac1表达变化，免疫荧光染色和Western blot分析内皮分化标志物，Matrigel凝胶实验观察血管索形成．结果表明，无论过表达或抑制Rac1的活化，并不影响EBs发育，均可形成典型的EBs胚层结构．抑制Rac1活化对内皮细胞系的发育无影响，但分化的内皮细胞不能连接成血管网．活化的Rac1表达减少，细胞迁移受到明显抑制．抑制Rac1活化导致细胞骨架F-actin排布紊乱．以上结果提示，Rac1影响胚胎早期血管发育的因素是抑制细胞游走，后者可能是通过F-actin机制所介导．
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| 关 键 词： | 胚胎干细胞 分化 血管发育 |
| 收稿时间： | 2008-10-07 |
| 修稿时间： | 2009-02-17 |
Inhibition of Rac1 activity hinders vasculogenesis during early embryonic development |
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| ZHANG Jian,HAN Ya-Ling,KANG Jian,ZHANG Xiao-Lin,QI Yan-Mei,YAN Cheng-Hui and LI Shao-Hua. Inhibition of Rac1 activity hinders vasculogenesis during early embryonic development[J]. Progress In Biochemistry and Biophysics, 2009, 36(6): 696-701. DOI: 10.3724/SP.J.1206.2008.00685 |
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| Authors: | ZHANG Jian HAN Ya-Ling KANG Jian ZHANG Xiao-Lin QI Yan-Mei YAN Cheng-Hui LI Shao-Hua |
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| Affiliation: | Department of Cardiology, Shenyang General Hospital, Cardiovascular Reseach Institute of PLA, Shenyang 110016, China;Department of Cardiology, Shenyang General Hospital, Cardiovascular Reseach Institute of PLA, Shenyang 110016, China;Department of Cardiology, Shenyang General Hospital, Cardiovascular Reseach Institute of PLA, Shenyang 110016, China;Department of Cardiology, Shenyang General Hospital, Cardiovascular Reseach Institute of PLA, Shenyang 110016, China;Department of Cardiology, Shenyang General Hospital, Cardiovascular Reseach Institute of PLA, Shenyang 110016, China;Department of Cardiology, Shenyang General Hospital, Cardiovascular Reseach Institute of PLA, Shenyang 110016, China;Department of Pathology and Laboratory Medicine, Robert Wood Johnson Medical School, New Jersey 08854, USA |
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| Abstract: | |
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| Keywords: | Rac1 |
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