| 短小芽孢杆菌的遗传转导 |
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| 引用本文： | 耿运琪,陈启民,崔立斌,蒋如璋. 短小芽孢杆菌的遗传转导[J]. 遗传学报, 1988, 0(6) |
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| 作者姓名： | 耿运琪 陈启民 崔立斌 蒋如璋 |
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| 作者单位： | 南开大学生物系生物工程研究室 天津(耿运琪，陈启民)，军事医学科学院基础医学研究所 研究生(崔立斌)，南开大学生物系生物工程研究室 天津(蒋如璋) |
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| 摘 要： | 短小芽孢杆菌(Bacillus pumilus)噬菌体PP5在碱性蛋白酶生产菌珠B.pumilus 289中能进行普遍性转导。PP5对于B.pumilus 289的营养标记的转导频率为10~(-6)转导子/PFU。对于B.pumilus 1037和B.pumilus 289之间的链霉素抗性标记的转导频率为10~(-4)至10~(-7)转导子/PFU。从而建立了一个新的B.pumilus遗传转导系统。
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| 关 键 词： | 噬菌体PP5 短小芽孢杆菌 普遍性转导 |
Genetic Transduction in Bacillus pumilus |
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| Geng Yunqi Chen Qimin Cui Libin Jiang Ruzhang. Genetic Transduction in Bacillus pumilus[J]. Journal of Genetics and Genomics, 1988, 0(6) |
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| Authors: | Geng Yunqi Chen Qimin Cui Libin Jiang Ruzhang |
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| Abstract: | Bacteriophage PP5 isolated from soil was able to mediate generalized transduction in Bacillus pumilus 289.The transduction frequency of auxotrophic markers of B.pumilus 289 was on the order of 10-6 transductants per PFU.The transduction frequency of Str2 (streptomycin-resistance) from B.fumilus 1037 to B.pumilus 289 was on the order of 10-6 to 10-7 |
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| Keywords: | Pbage PP5 Bacillus fumilus Generalized transduction |
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