An enhanced miniaturized assay for antimicrobial prospecting |
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Authors: | Smith Shannon M Beattie Andrew J Gillings Michael R Holley Marita P Stow Adam J Turnbull Christine L Wilson Peter D Briscoe David A |
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Institution: | Department of Biological Sciences, Macquarie University, NSW, Australia. smsmith@bio.mq.edu.au |
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Abstract: | Bioprospecting for novel antimicrobials increasingly relies on extremely small samples unsuitable for conventional bulk extraction and assay. We developed a microtitre plate assay for minimal amounts of test materials which is rapid, extremely sensitive, allows time-course analysis and reduces false negatives. Developed for the analyses of antimicrobial sensitivity and resistance, the technique is appropriate for assays where source materials are scarce. |
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