| 一株猴泡沫病毒野毒Pol基因的cDNA的直接测序 |
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| 引用本文： | 唐东红,江勤芳,匡德宣,代解杰. 一株猴泡沫病毒野毒Pol基因的cDNA的直接测序[J]. 中国实验动物学报, 2000, 8(1): 22-25 |
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| 作者姓名： | 唐东红 江勤芳 匡德宣 代解杰 |
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| 作者单位： | 中国医学科学院,中国协和医科大学,医学生物学研究所,昆明花红洞,650107 |
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| 基金项目： | 云南省中青年学术技术带头人基金 |
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| 摘 要： | A 377 Kunming cell line of Rhesus Monkey Kidney (RMK) fromCentre for Medical Pr imates was amplified the cDNA fragments of the pol gene byNested-PCR,which of a natured infected SFV showed typical CPE after cultured for threeweeks,sequence s of products position(nt 5989-6343)were determined directly.The resultindicate d that the nucleotid sequences in the region had 48 nucleotids difference(10.78%) between SFV infected cell and SFV-1 in GENE BANK,and there was 45 nucleotidesdifference(10.11%) compared with SFVmac in the region, the test with VspⅠ showe d aligment.
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| 关 键 词： | 猴泡沫病毒 序列测定 |
The cDNA Nested-PCR Direct Sequencing pol Region of A Simian Foamy Virus in Rhesus Monkey |
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| Tang Dong-hong,Jiang Qin-fang,Kuang De-xuan,Dai Jie-jie. The cDNA Nested-PCR Direct Sequencing pol Region of A Simian Foamy Virus in Rhesus Monkey[J]. Acta Laboratorium Animalis Scientia Sinica, 2000, 8(1): 22-25 |
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| Authors: | Tang Dong-hong Jiang Qin-fang Kuang De-xuan Dai Jie-jie |
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| Abstract: | |
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| Keywords: | Simian Foamy Virus(SFV) Sequencing |
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