Insertional inactivation of a gene which controls expression of vancomycin resistance on plasmid pHKK100 |
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| Authors: | Sandra Handwerger Linda Discotto Jane Thanassi Michael J. Pucci |
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| Affiliation: | Division of Infectious Diseases, Beth Israel Medical Center, Mount Sinai School of Medicine, New York, NY. |
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| Abstract: | Expression of inducible high level vancomycin resistance (Vmr) in enterococci appears to require other plasmid-encoded genes in addition to the previously described structural genes vanA and vanH. Tn917 mutagenesis was used to identify such a region in the Vmr plasmid pHKK100. Insertional inactivation of a 693-bp open reading frame upstream from vanH resulted in complete loss of Vmr. This putative 26,642-Da protein has been designated VanR. |
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| Keywords: | Gene vanA Vancomycin resistance Plasmid pHKK100 |
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