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High efficiency temperature-sensitive amber suppressor strains of Escherichia coli K12: Isolation of strains with suppressor-enhancing mutations |
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| Authors: | Max P. Oeschger Nicole S. Oeschger Grant T. Wiprud Sharon Lee Woods |
| Affiliation: | (1) Department of Microbiology, Georgetown University School of Medicine, 20007 Washington, D.C., USA;(2) Georgetown University School of Dentistry, 20007 Washington, D.C., USA;(3) Present address: Department of Human Genetics, Yale University, 06510 New Haven, Connecticut, USA |
| Abstract: | Summary Two independent high efficiency ts amber suppressor strains have been isolated as derivatives of a well-characterized supD ts suppressor strain (Oeschger and Woods, 1976). The mutations which raise suppressor activity have been shown by Hfr mapping to be distinct from each other and the supD locus. One of the isolates provides up to 100% efficient suppressor activity at low temperatures. |
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