Inhibition of the mixed lymphocyte culture by peritoneal exudate cells. |
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Authors: | B R Fernbach H Kirchner R B Herberman |
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Affiliation: | Laboratory of Cell Biology and Laboratory of Immunodiagnosis, National Cancer Institute, Bethesda, Maryland 20014 U.S.A. |
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Abstract: | Inhibition of mixed lymphocyte cultures (MLC) by macrophages and by supernatants of short term cultured macrophages was assessed by incorporation of 3H-thymidine (TdRH3) and also by blast cell counts and by determination of cellmediated lympholysis. Peritoneal exudate cells (PEC) induced by thioglycollate, at concentrations >10%, inhibited all three parameters of MLC. Lower concentrations of PEC, and supernatants from cultured PEC, inhibited TdRH3 incorporation, but had no significant effect on blast cell counts or on generation of cytotoxic effector cells. Inhibition by the supernatants could be reversed by dialysis or by use of low specific activity TdRH3. These data indicate that macrophages can inhibit proliferative responses in MLC, but that this must be carefully distinguished from selective inhibition of TdRH3 incorporation. |
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Keywords: | Address reprint requests to: Dr. Ronald B. Herberman Laboratory of Immunodiagnosis National Cancer Institute Building 8 Room 118 Bethesda Maryland 20014 USA. |
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