Nonpermissiveness for mouse embryonic stem (ES) cell derivation circumvented by a single backcross to 129/Sv strain: establishment of ES cell lines bearing the Om
d
conditional lethal mutation |
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Authors: | Chantal Kress Sandrine Vandormael-Pournin Patricia Baldacci Michel Cohen-Tannoudji Charles Babinet |
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Institution: | (1) Unité de Biologie du Développement, URA C.N.R.S. 1960, Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris Cedex 15, France, FR |
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Abstract: | The inbred mouse strain DDK carries a conditional early embryonic lethal mutation that is manifested when DDK females are
crossed to males of other inbred strains but not in the corresponding reciprocal crosses. It has been shown that embryonic
lethality could be assigned to a single genetic locus called Ovum mutant (Om), on Chromosome (Chr) 11 near Syca 1. In the course of our study of the molecular mechanisms underlying the embryonic lethality, we were interested in deriving
an embryonic stem cell bearing the Om mutation in the homozygous state (Om
d
/Om
d
). However, it turned out that DDK is nonpermissive for ES cell establishment, with a standard protocol. Here we show that
permissiveness could be obtained using Om
d
/Om
d
blastocysts with a 75% 129/Sv and 25% DDK genetic background. Several germline-competent Om
d
/Om
d
ES cell lines have been derived from blastocysts of this genotype. Such a scenario could be extended to the generation of
ES cell lines bearing any mutation present in an otherwise nonpermissive mouse strain.
Received: 2 June 1998 / Accepted: 18 August 1998 |
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Keywords: | |
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