Survival of rat functional dental pulp cells in vascularized tissue engineering chambers |
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Authors: | Srisuwan T Tilkorn D J Al-Benna S Vashi A Penington A Messer H H Abberton K M Thompson E W |
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Institution: | a Department of Restorative Dentistry and Periodontology, Faculty of Dentistry, Chiang Mai University, Chiang Mai 50200, Thailand b Department of Plastic Surgery, Burn Center, Hand Center, Sarcoma Reference Center, BG-University-Hospital Bergmannsheil, Ruhr-University Bochum, Buerkle-de-la Camp Platz 1, 44789 Bochum, Germany c CSIRO Materials Science and Engineering, Bayview Avenue, Clayton, Victoria 3168, Australia d School of Dental Science, Department of Restorative Dentistry, The University of Melbourne, 720 Swanston Street, Melbourne, Victoria 3010, Australia e The O’Brien Institute of Microsurgery, 42 Fitzroy Street, Fitzroy, Victoria 3065, Australia |
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Abstract: | Regenerative endodontics aims to preserve, repair or regenerate the dental pulp tissue. Dental pulp stem cells, have a potential use in dental tissue generation. However, specific requirements to drive the dental tissue generation are still obscured. We established an in vivo model for studying the survival of dental pulp cells (DPC) and their potential to generate dental pulp tissue. DPC were mixed with collagen scaffold with or without slow release bone morphogenic protein 4 (BMP-4) and fibroblast growth factor 2 (FGF2). The cell suspension was transplanted into a vascularized tissue engineering chamber in the rat groin. Tissue constructs were harvested after 2, 4, 6, and 8 weeks and processed for histomorphological and immunohistochemical analysis. After 2 weeks newly formed tissue with new blood vessel formation were observed inside the chamber. DPC were found around dentin, particularly around the vascular pedicle and also close to the gelatin microspheres. Cell survival, was confirmed up to 8 weeks after transplantation. Dentin Sialophosphoprotein (DSPP) positive matrix production was detected in the chamber, indicating functionality of dental pulp progenitor cells. This study demonstrates the potential of our tissue engineering model to study rat dental pulp cells and their behavior in dental pulp regeneration, for future development of an alternative treatment using these techniques. |
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Keywords: | Dental pulp cells Dental pulp stem cells Regenerative endodontics Tissue regeneration Tissue engineering Vascularized tissue engineering chamber Odontoblast Dentin Dentin Sialophosphoprotein Basic fibroblast growth factors Bone morphogenic protein-4 |
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