Hormonal stimulation of maturation and ovulation,gamete morphology,and raising of larvae in Dascyllus trimaculatus (Pomacentridae)

The experiments on hormonal stimulation of the maturation and ovulation of oocytes of Dascyllus trimaculatus (Pomacentridae) are carried out. Double injections of surfagon (LH-RH-a) (5 + 15 μg/kg of fish body weight) with (or without) the addition of eglonil (5 + 15 mg/kg) are used, and the interval between the injections ranges from 12 to 17 h. Ovulation is registered 33.5–42.0 h after injection I. Morphological changes in oocytes are followed during the process of their maturation. The ultrastructure of spermatozoa and oocyte envelopes is studied. The quality of ovulated oocytes is assessed after their in vitro storage at 25 and 5°C. Preliminary results on the transition of the larvae to exogenous feeding are obtained.