| 双标记RC对KHT肉瘤的细胞周期分析(英文) |
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| 引用本文： | 薛绍白,M.巴拉维西尼,J.格瑞. 双标记RC对KHT肉瘤的细胞周期分析(英文)[J]. 分子细胞生物学报, 1985, 0(2) |
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| 作者姓名： | 薛绍白 M.巴拉维西尼 J.格瑞 |
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| 作者单位： | 北京师范大学生物系(薛绍白)，美国劳伦茨利·温摩尔国家实验室(M.巴拉维西尼)，美国劳伦茨利·温摩尔国家实验室(J.格瑞) |
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| 基金项目： | This work was performed under the auspices of the United states Department of Energy by the Lawrence Livermore National Laberatory under contract number W-7405-ENG-48 with support from USPHS grant CA 14533 |
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| 摘 要： | 本文利用测定G_1期和中S期细胞内放射性变化的方法(RC)测出小鼠KHT肉瘤的细胞周期时相的时间及其变异系数(CV)。腹腔注射~3H-UdR后,8小时再注射~(125)I-UdR,按2小时间隔取肿瘤制成单个细胞悬液,DNA特异性染料色霉素A_3染色,根据细胞DNA含量用FACS荧光激活细胞分类器分离出纯的G_1期和中S期细胞,分别测定细胞中~(125)I和~3H的放射性,用多室数学模型根据每个细胞内~(125)I和~3H的放射性变化,计算出TG_1为6.7小时,Ts为9.0小时,TG2M为3小时,生长指数为1。
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DOUBLE LABEL RADIOACTIVITY PER CELL(RC)ANALYSIS IN VIVO:RAPID CYTOKINETIC ANALYSIS OF THE KHT SARCOMA |
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| XUE SHAO BAI. DOUBLE LABEL RADIOACTIVITY PER CELL(RC)ANALYSIS IN VIVO:RAPID CYTOKINETIC ANALYSIS OF THE KHT SARCOMA[J]. Journal of Molecular Cell Biology, 1985, 0(2) |
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| Authors: | XUE SHAO BAI |
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| Abstract: | We have used a double labelradioactivity per cell (RC) analysisprocedure to rapidly estimate the G1,S-and G2M phase durations (andtheir coeficients of variation) of amurine solid tumor in vivo.We injectedtumor-bearing mice with 3~H-deoxyuri-dine at time 0 and 8 hrs laterwith (125)~I-deoxyuridine.Tumors wereharvested after 30 min and at two hourintervals for an additional 12 hours.The tumors were dispersed into a singlecell suspension,stained with a DNA-specific dye and cells in G1 and mid-S-phase sorted for radioactivity measure-ments.The variations in radioactivityper cell were analyzed using a multi-compartment mathematical model toobtain phase durations and tumor grow-th fraction.The G1,S-and G2Mphase durations of the KHT tumorwere found to be 6.7,9.0 and 3.0 hrs,respectively.The growth fraction wasunity. The double label RC analysis,technique is significantly faster andpotentially more accurate than autora-diographic techniques for phase du-ration estimation.Its application tocytokinetic analysis of a solid tumorindicates that it will be a useful tool torapidly estimate cytokinetic proper-ties of tissues in vivo. |
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| Keywords: | Double label RC analysis technique.KHT sercoma.in vivo. |
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