| REMI介导电转化产甘油假丝酵母 |
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| 引用本文： | 张永光,沈微,饶志明,方慧英,诸葛健.REMI介导电转化产甘油假丝酵母[J].微生物学通报,2007,34(5):0848-0851. |
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| 作者姓名： | 张永光 沈微 饶志明 方慧英 诸葛健 |
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| 作者单位： | 江南大学生物工程学院工业微生物研究中心,工业生物技术教育部重点实验室,无锡,214036 |
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| 基金项目： | 江苏省青年科技创新基金;国家自然科学基金 |
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| 摘 要： | 为了分离耐高渗和甘油代谢相关基因,以Zeocin为选择标记,利用REMI技术电转化产甘油假丝酵母Candida glycerinogenes。考察了7种限制性内切酶对转化的影响,选择HindIII进一步优化了转化的几个条件。结果表明,在OD600≈1.3时收集细胞,在1.5kV电压下,感受态细胞浓度为2.0×109个细胞/mL,100U Hind III时,能获得129个转化子/μgDNA的较高转化率,58%的转化子稳定,表明REMI技术适合于产甘油假丝酵母的转化。
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| 关 键 词： | 产甘油假丝酵母 遗传转化 |
| 文章编号： | 0253-2654（2007）05-0848-04 |
| 修稿时间： | 2006-09-19 |
Genetic Transformation of Candida glycerinogenes by REMI and Electroporation |
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| ZHANG Yong-Guang,SHEN Wei,RAO Zhi-Ming,FANG Hui-Ying and ZHUGE Jian.Genetic Transformation of Candida glycerinogenes by REMI and Electroporation[J].Microbiology,2007,34(5):0848-0851. |
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| Authors: | ZHANG Yong-Guang SHEN Wei RAO Zhi-Ming FANG Hui-Ying and ZHUGE Jian |
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| Institution: | Research Center of Industrial Microbiology, School of Biotechnology, the Key Laboratory of Industrial Biotechnology, Ministry of Education, Southern Yangtze University,Wuxi 214036;Research Center of Industrial Microbiology, School of Biotechnology, the Key Laboratory of Industrial Biotechnology, Ministry of Education, Southern Yangtze University,Wuxi 214036;Research Center of Industrial Microbiology, School of Biotechnology, the Key Laboratory of Industrial Biotechnology, Ministry of Education, Southern Yangtze University,Wuxi 214036;Research Center of Industrial Microbiology, School of Biotechnology, the Key Laboratory of Industrial Biotechnology, Ministry of Education, Southern Yangtze University,Wuxi 214036;Research Center of Industrial Microbiology, School of Biotechnology, the Key Laboratory of Industrial Biotechnology, Ministry of Education, Southern Yangtze University,Wuxi 214036 |
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| Abstract: | |
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| Keywords: | REMI |
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