Two-photon induced fluorescence of Cy5-DNA in buffer solution and on silver island films |
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| Authors: | Lukomska Joanna Gryczynski Ignacy Malicka Joanna Makowiec Slawomir Lakowicz Joseph R Gryczynski Zygmunt |
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| Institution: | Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland at Baltimore, 725 West Lombard Street, Baltimore, MD 21201, USA. joanna@cfs.umbi.umd.edu |
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| Abstract: | We report the observation of a strong two-photon induced fluorescence emission of Cy5-DNA within the tunable range of a Ti:Sapphire laser. The estimated two-photon cross-section for Cy5-DNA of 400GM is about 3.5-fold higher than it was reported for rhodamine B. The fundamental anisotropies of Cy5-DNA are close to the theoretical limits of 2/5 and 4/7 for one- and two-photon excitation, respectively. We also observed an enhanced two-photon induced fluorescence (TPIF) of Cy5-DNA deposited on silver island films (SIFs). In the presence of SIFs, the TPIF is about 100-fold brighter. The brightness increase of Cy5-DNA TPIF near SIFs is mostly due to enhanced local field. |
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| Keywords: | Two-photon excitation Cy5 Silver islands |
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