Ethanol tolerance, yield of melanin, swarming motility and growth are correlated with the expression levels of aiiA gene in Bacillus thuringiensis |
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Authors: | Yi Zhou Wei-Xing Ye Ying Zhou Chen-Guang Zhu Ming Sun Zi-Niu Yu |
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Affiliation: | aState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China bChangjiang University, Jingzhou 434025, China |
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Abstract: | To investigate the role of aiiA gene in Bacillus thuringiensis, B. thuringiensis strain BMB171 was selected and three derivatives were constructed, such that (1) aiiA was knocked out (aiiA-KO), (2) aiiA-KO was reversed (aiiA-Rev) and (3) the expression of aiiA was elevated (aiiA-Elevated). First, we found that mutation of aiiA increased the ethanol tolerance in B. thuringiensis. Omitting ethanol from the medium resulted in a decreased generation time for aiiA-Elevated. In contrast, aiiA-KO had the highest ethanol tolerance when the medium contained 2 or 4% ethanol. Second, we found that more melanin was produced at 37 °C when aiiA expression was elevated. Third, we found inhibition of swarming motility in the aiiA-Elevated group. Finally, there was delayed sporulation in Luria–Bertani, ICPM, casein and ammonium sulfate medium in the aiiA-Elevated group. The conclusion was that ethanol tolerance, yield of melanin, swarming motility and growth are correlated with the expression levels of aiiA gene in B. thuringiensis. |
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Keywords: | B. thuringiensis AHL lactonase Melanin Ethanol tolerance Growth Swarming motility |
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