An investigation into thee mechanism of citrate---FE-dependent lipid peroxidation |
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Authors: | Giorgio Minotti and Steven D. Aust |
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Affiliation: | Center for the Study of Active Oxygen in Biology and Medicine, Department of Biochemistry, Room 215 Biochemistry Building, Michigan State University, East Lansing, MI 48824-1319, USA |
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Abstract: | Chelation by citrate was found to promote the autoxidation of Fe2+, measured as the disapperance of 1,10-phenanthroline-chelatable Fe2+. The autoxidation of citrate---2+ could in turn promote the peroxidation of microsomal phospholipid liposomes, as judged by malondialdehyde formation. At low citrate---Fe2+ ratios the autoxidation of Fe2+ was slow and the formation of malondialdehyde was preceded by a lag phase. The lag phase evidence of this, linear initial rates of lipid peroxidation were obtained via the combination of citrate---Fe2+ and citrate---Fe3+, optimum activity occurring at a Fe3+---Fe2+ ratio of 1:1. Evidence is also presented to suggest that the superoxide and the hydrogen peroxide that are formed during the autoxidation of citrate---Fe2+ can either stimulate or inhibit lipid peroxidation by affecting the yield of citrate---Fe3+ from citrate---Fe2+. No evidence was obtained for the participation of the hydroxyl radical in the initiation of lipid peroxidation by citrate---Fe2+. |
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Keywords: | Citrate Ferrous Chelation Autoxidation Fe3+:Fe2+ ratio Lipid peroxidation |
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