Low- to high-throughput analysis of telomerase modulators with Telospot |
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| Authors: | Cristofari Gaël Reichenbach Patrick Regamey Pierre-Olivier Banfi Damiano Chambon Marc Turcatti Gerardo Lingner Joachim |
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| Institution: | Swiss Institute for Experimental Cancer Research, Chemin des Boveresses 155, CH-1066 Epalinges, Switzerland. |
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| Abstract: | We designed a method termed Telospot to discover and characterize telomerase modulators as anticancer drugs or chemical biology tools. Telospot is based on a highly efficient human telomerase expression system and the detection of telomerase DNA reaction products in macroarray format. Telospot offers a highly scalable, cost- and time-effective alternative to presently available telomerase assays, which are limited by the requirement for PCR, telomerase purification or technologies not amenable to high throughput. |
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