PCR amplification of the rDNA internal transcribed spacer region for differentiation of Saccharomyces cultures |
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| Authors: | Patricia Valente Fábio C Gouveia Glauber A de Lemos Douglas Pimentel Jan D van Elsas Leda C Mendonça-Hagler Allen N Hagler |
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| Institution: | Institute de Microbiologia Prof. Paulo de Goes, CCS, Bloco I, UFRJ, Ilha do Fundão, Rio de Janeiro, CEP 21941-590, Brazil; Programa de Pós-Graduação em Biotecnologia Vegetal, CCS, UFRJ, Rio de Janeiro, Brazil; Programa de Pós-Graduação em Ecologia, Instituto de Biologia, UFRJ, Rio de Janeiro, Brazil; Institute for Soil Fertility Research IB-DLO P.O. Box 9060, 6700 GW Wageningen, The Netherlands |
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| Abstract: | Abstract The size of the internal transcribed spacer (ITS) region as measured by gel electrophoresis of PCR products, amplified by primers ITS1 and ITS4, was over 800 bp for all Saccharomyces sensu stricto species, but yeasts belonging to other Saccharomyces species had a shorter ITS region, making this characteristic potentially useful in the identification of Saccharomyces isolates. The ITS product length was homogeneous within the species Saccharomyces cerevisiae . |
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| Keywords: | Saccharomyces PCR Internal transcribed spacer Yeast taxonomy |
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